inhibition of human erythrocyte glucose 6-phosphate dehydrogenase by cadmium, nickel and aluminium

Inhibition of Human Erythrocyte Glucose 6-Phosphate Dehydrogenase by Cadmium, Nickel and Aluminium

Inhibition of Human Erythrocyte Glucose 6-Phosphate Dehydrogenase by Cadmium, Nickel and Aluminium

The inhibition of human erythrocyte glucose 6-phosphate dehydrogenase (G6PD) by Cd (II), Al (III) and Ni (II) was studied. The enzyme was partially purified having specific activity of 1.4 U/mg protein. Cadmium inhibited G6PD activity progressively up to 1.5 mM concentration where about 65% of the enzyme activity was lost. The inhibition was uncompetitive and noncompetitive with respect to gluc...

INHIBITION OF HUMAN ERYTHROCYTE GLUCOSE 6-PHOSPHATE DEHYDROGENASE ACTIVITY BY DEHYDROEPIANDROSTERONE AND RELATED STEROIDS.

The inhibitory effects of several steroids on G6PD activity using intact erythrocytes are reported. Incubation of whole blood with dehydroepiandrosterone (DHEA) resulted in 42% and 12% inhibition in the enzyme activity in the presence and absence of oxygen, respectively. Addition of epinephrine and/or aminophylline into the incubation medium caused further enzyme inhibition suggesting a po...

inhibition of human erythrocyte glucose 6-phosphate dehydrogenase activity by dehydroepiandrosterone and related steroids.

the inhibitory effects of several steroids on g6pd activity using intact erythrocytes are reported. incubation of whole blood with dehydroepiandrosterone (dhea) resulted in 42% and 12% inhibition in the enzyme activity in the presence and absence of oxygen, respectively. addition of epinephrine and/or aminophylline into the incubation medium caused further enzyme inhibition suggesting a possibl...

Genetic Variants of Human Erythrocyte Glucose-6-phosphate Dehydrogenase

The values of V,,,,, and K, for the three genetic variants A, B, and Aof erythrocyte glucose-&phosphate dehydrogenase have been determined at 10 different pH values in the range from 5.5 to 9.5, and at four different temperatures in the range from 18.5-40.0”. The log V,,,,, versus pH curve for each of the enzymes shows a monotonic increase between pH 5.5 and 7, and a plateau from pH 7.5 upwards...

incidence of erythrocyte glucose-6-phosphate- dehydrogenase deficiency

in a survey carried out to establish the incidence of erythrocyte glucose­ 6-phosphate-dehydrogenase deficiency, 738 professional blood donors of tehran were examined. the fluorescent spot technique was used for screening and qualitative determination of g-6-pd in erythrocytes. this technique was compared with other methods of g-6-pd enzyme assay and proved to be very reliable. qualitative enzy...